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Grow yeast experiment science experiment : Fizzics Education

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Grow yeast experiment

Grow yeast experiment

Follow FizzicsEd 150 Science Experiments:

You Will Need:

  • 4 packets of dry yeast
  • 4 water bottles, chilled in the fridge (we use Thank You Water, a social enterprise that works to get
    clean water & sanitation to people in need)
  • 4 balloons
  • 1 funnel.
  • 1 spoon.
  • 1 large jug.
  • 4 measuring cups.
  • 4 thermometers (one will do if you don’t have a class set).
  • Sugar.
  • Access to boiling water plus adult supervision.
  • 1 stopwatch.
  • A pen to mark the water temperature on each water bottle during the experiment.
  • A shelf to leave the science experiment to run.
  • A notebook for your observations.

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Yeast growth science experiment - materials needed
1 Yeast growth science experiment - taking temperature readings of the waters

Pour out the 4 chilled water bottles into the large jug and discard the rest of the water (maybe water your school garden!)

Carefully measure out the water into the four measuring cups as per the measurements below;

Cup 1 – 200mL of chilled water

Cup 2 – 150mL of chilled water

Cup 3 – 100mL of chilled water

Cup 4 – 50mL of chilled water

Use the thermometers to take a measurement of the water temperature in each cup (write this in your notebook).

With an adult, boil a jug of water and then top up cups 2, 3 and 4 so that they too have 200mL of water as per cup 1. You will be testing the effect of temperature on the growth of yeast by measuring how much gas is released by the yeast under 4 different temperature conditions (variable testing).

 

2 Yeast growth science experiment - adding yeast to a water bottle

Using a funnel, carefully pour each cup of water into the four separate water bottles. Use the pen to mark the starting temperature of each water bottle.

3 Yeast growth science experiment - adding sugar to water in a bottle

Add a spoonful of sugar per water bottle and then swirl the bottle to dissolve the sugar.

4 Yeast growth science experiment - labelled bottles at start of activity

Add a yeast packet into each bottle and quickly stretch a balloon of the opening of each bottle.

5 Yeast growth science experiment - final result

4 yeast growth experiments started, showing a distinct change already!

Start the stopwatch and take notes of when each balloon rises!

OPTIONAL: you could also keep each bottle in the yeast experiment at the same temperature and vary the amount of sugar added instead.

6 4 student worksheets on a yeast fermentation experiment
7 Microscope
8 Teacher showing how to do an experiment outside to a group of kids.

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What is going on?

Your experiment was testing the effect of water temperature on the growth of yeast. Yeast are egg-shaped microscopic cells of fungi that are dormant whilst kept in dry and cool conditions. However, yeast will rapidly divide once exposed to water and sugar in ideal temperatures. In the right temperature, yeast cells will change the sugar into glucose by using the water plus as an enzyme catalyst (invertase). Once the yeast has converted the sugar to glucose fermentation can then occur to produce carbon dioxide and ethanol as per the equation below;

Glucose ⟶ Ethanol + Carbon dioxide

which can be written as…

C6H12O6(aq)  ⟶ 2C2H5OH(aq) + 2CO2(g)

In your experiment, you were trapping the carbon dioxide released during the fermentation process. The more active the yeast, the more carbon dioxide the yeast produced! In your experiment, the different water temperatures will have produced different results as bottles may have been too hot for the yeast to survive whereas the other bottles may have been too cold.  By introducing a variable to test in your experiment, you’re doing real science! The following list of temperatures is worth keeping in mind when assessing your results:

  • 55° C – 60° C
    Yeast cells die (also known as the thermal death point).
  • 41° C – 46° C
    Ideal temperature of water for dry yeast being reconstituted with water and sugar.
  • 4° C
    The temperature of a fridge – yeast will be too cold to work properly.

Yeast is used to make bread rise and to ferment beer. There are many different species of yeast, but the one most commonly used in cooking and baking is called Saccharomyces cerevisiae, which is also known as brewer’s yeast.

Yeast
Yeast we used for our science experiment.

Yeast can break down many types of simple carbohydrates (monosaccharides) however they cannot break down complex carbohydrates such as starch. This means that extra enzymes are needed to break down starch into sugars that the yeast can use, for example during beer production we use enzymes from germinating barley to do this.

Variables to test

More on variables here

  • Try different concentrations of vinegar as the growth medium. Can the yeast handle some acidity?
  • Vary the amount of sugar used.
  • Does the volume of water make a difference?

Classroom activity sheets for this experiment

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4 student worksheets on a yeast fermentation experiment

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Comments

4 thoughts on “Grow yeast experiment

    1. Hi!
      Here’s the detail’s that you need;

      > Cup 1 – 200mL of chilled water
      > Cup 2 – 150mL of chilled water
      > Cup 3 – 100mL of chilled water
      > Cup 4 – 50mL of chilled water

      Use the thermometers to take a measurement of the water temperature in each cup (write this in your notebook).

      With an adult, boil a jug of water and then top up cups 2, 3 and 4 so that they too have 200mL of water as per cup 1. You will be testing the effect of temperature on the growth of yeast by measuring how much gas is released by the yeast under 4 different temperature conditions.

  1. Would this experiment still work if instead i tested how different types of sugars affect the amount of fermentation by yeast. Would i still get different sized balloons in my result.

    1. We’d love it if you try this and let us know!
      With any experiment you just have to change one thing and then measure the result.
      So, changing the types of sugars is a completely valid investigation.
      Good luck!

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